Journal: The Journal of nutritional biochemistry

Article Title: Resveratrol prevents the development of choroidal neovascularization by modulating AMP-activated protein kinase in macrophages and other cell types.

doi: 10.1016/j.jnutbio.2014.05.015

Figure Lengend Snippet: Fig. 3. RSV treatment reduced the levels of inflammatory and angiogenic molecules.Protein levels of VEGF (A), ICAM-1 (B) and MCP-1 (C) were increased 3 days after CNV induction. RSV pretreatment significantly suppressed these increases. n=8. **Pb.001, *Pb.01, †Pb.05.

Article Snippet: In the samples taken 3 days after the PC, VEGF, MCP-1 and ICAM-1 levels were determined with mouse VEGF, MCP-1 and ICAM-1 ELISA kits (R&D Systems, Minneapolis, MN, USA) as previously described [10].

Techniques:

Journal: The Journal of nutritional biochemistry

Article Title: Resveratrol prevents the development of choroidal neovascularization by modulating AMP-activated protein kinase in macrophages and other cell types.

doi: 10.1016/j.jnutbio.2014.05.015

Figure Lengend Snippet: Fig. 5. RSV inhibited NF-κB and inflammatory and angiogenic molecules in vitro. ARPE-19 cells (A–C), b-End3 microvascular endothelial cells (D–F) and RAW264.7 macrophages (G and H) were stimulated with TNF-α. RSV significantly suppressed the phosphorylation (activation) of NF-κB by TNF-α (A, D and G) and the subsequent induction of VEGF (B and H), MCP-1 (C and F) and ICAM-1 (E). All of the effects of RSV were attenuated by an AMPK inhibitor, compound C. n=8. **Pb.001, *Pb.01, †Pb.05.

Article Snippet: In the samples taken 3 days after the PC, VEGF, MCP-1 and ICAM-1 levels were determined with mouse VEGF, MCP-1 and ICAM-1 ELISA kits (R&D Systems, Minneapolis, MN, USA) as previously described [10].

Techniques: In Vitro, Phospho-proteomics, Activation Assay

Journal: The Journal of nutritional biochemistry

Article Title: Resveratrol prevents the development of choroidal neovascularization by modulating AMP-activated protein kinase in macrophages and other cell types.

doi: 10.1016/j.jnutbio.2014.05.015

Figure Lengend Snippet: Fig. 7. A model for the preventive effects of RSV on CNV.RSV treatment significantly suppresses CNV development by preserving AMPK activation, which inhibits macrophage recruitment as well as the induction of inflammatory processes, including NF-κB activation and the subsequent up-regulation of inflammatory and angiogenic molecules such as ICAM-1, MCP-1 and VEGF in multiple cell types. PC, laser PC for CNV induction; Vascular EC, vascular endothelial cells.

Article Snippet: In the samples taken 3 days after the PC, VEGF, MCP-1 and ICAM-1 levels were determined with mouse VEGF, MCP-1 and ICAM-1 ELISA kits (R&D Systems, Minneapolis, MN, USA) as previously described [10].

Techniques: Preserving, Activation Assay

Journal: Cancer Science

Article Title: Intraperitoneal administration of nanoparticles containing tocopheryl succinate prevents peritoneal dissemination

doi: 10.1111/cas.15321

Figure Lengend Snippet: Improvement in intraperitoneal microenvironment by TS‐NP. (A) The volume of ascites, (B, D) levels of VEGF‐A and IL‐10 and (C) CD163 levels as M2 marker in control and TS‐NP administered mice. Values and bars represent means and SD, respectively. n = 3–5, * p < 0.05 and *** p < 0.001. IL‐10, interleukin 10; TS‐NP, tocopheryl succinate nanoparticles; VEGF‐A, vascular endothelial growth factor A

Article Snippet: Vascular endothelial growth factor A (VEGF‐A), interleukin 10 (IL‐10), and tumor necrosis factor alpha (TNF‐α) levels in blood were determined using the Mouse VEGF Quantikine ELISA kit (R&D Systems, Inc., MN, USA) and Mouse IL‐10 ELISA Kit (ThermoFisher Scientific Inc. (IBM Corp., MA, USA), respectively.

Techniques: Marker, Control

Journal:

Article Title: Evidence for the importance of angiotensin II type 1 receptor in ischemia-induced angiogenesis

doi: 10.1172/JCI13055

Figure Lengend Snippet: Impacts of the implantation of WT mouse– or AT1a–/– mouse–derived MNCs into the ischemic hindlimbs of AT1a–/– mice on angiogenesis. The impaired LDBF ratio in AT1a–/– mice was partially but significantly restored after implantation of WT mouse–derived MNCs into the ischemic hindlimb of AT1a–/– mice (P < 0.01 vs. AT1a–/–). In contrast, implantation of AT1a–/– mouse–derived MNCs slightly improved the LDBF ratio in AT1a–/– mice, but the difference was not significant (vs. AT1a–/–). Neutralizing anti-VEGF mAb treatment abolished the WT-MNC–mediated rescue of impaired angiogenesis in AT1a–/– mice (P = NS vs. AT1a–/–).

Article Snippet: In five additional mice, neutralizing anti-mouse VEGF mAb (R&D Systems Inc., Minneapolis, Minnesota, USA) was continuously administered by a subcutaneously implanted osmotic pump (ALZA Corp.), and ischemia-induced angiogenesis was examined in AT1a –/– mice that had been subjected to WT-derived MNC transplantation.

Techniques: Derivative Assay